Fig. 6.

Gene expression analysis in HAT from male and female samples using different HKGs. A Coefficient of variation (CV) in the Cp values of each candidate gene calculated in male and female for lean and obese samples. B IRS1, LEPR, and PPARγ expression analysis using PPIA, RPL19, and 18S as reference genes. Male Lean n = 3; Female Lean n = 7; Male Obese n = 10; Female Obese n = 10. Student's t-test applied for significance—*p-value < 0.05, and **p-value < 0.01