Table 3.
Candidate suHKGs for gene expression analysis
| Gene | Relative stability in male | Relative stability in female | Expression level (TPM) |
Expression level (TPM) in female | Expression level (TPM) in male |
|---|---|---|---|---|---|
| PPIA | 873 | 1589 | 234.597 | 236.1 | 233.6 |
| RPL19 | 1129.67 | 137.33 | 1707.61 | 1707 | 1708 |
| RPS8 | 194.67 | 178.33 | 952.191 | 944.5 | 957.9 |
| RPS18 | 119.33 | 296.33 | 3173.82 | 3180 | 3168 |
| UBB | 228 | 79 | 252.293 | 249.8 | 254.1 |
| UBC | 267.33 | 706.33 | 432.547 | 396.9 | 447.7 |
Selection of housekeeping candidate genes proposed to be used as a reference to compare both sexes in gene expression analysis
PPIA and RPL19 have been experimentally validated, while RPS8, RPS18, UBB, and UBC are computationally suggested
These genes are proposed based on their sex-specific values of relative expression stability obtained from the final MetaRanking positions
Expression levels have been extracted from GTEx (given in TPM), which are high enough for detection by different technologies