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Antibodies used for immunofluorescence microscopy.
| Antibodies | Cat # | Final concentration | Dilution | Manufacturer |
|---|---|---|---|---|
| NP | EBS-I-047 | 2.0 μg/ml | 1:1,000 | EVL Laboratories |
| VE-CAD | AF938 | 2.0 μg/ml | 1:100 | R&D Systems |
| Acetylated-α-tubulin | SC-23950 | 2.0 μg/ml | 1:100 | Santa Cruz Biotechnology |
| ZO-1 | 339100 | 5.0 μg/ml | 1:100 | Life Technologies/Invitrogen |
| Hoechst | 62249 | 2 μM | 1:10,000 | Life Technologies/Invitrogen |
