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. 2023 Apr 18;6(7):e202302005. doi: 10.26508/lsa.202302005

Figure 1. Forward genetic screen of the haploid mouse embryonic stem cell library for resistance to RVFV MP-12.

Figure 1.

(A) Schematic representation of the retroviral revertible genetrap used for mutagenesis. BC, barcode; OPE, Oct4 binding sites; SA, splicing acceptor site. (B) Experimental workflow for the RVFV MP-12 resistance screen using insertional mutagenesis. Bright-field microscopy images of the cells before infection and at the end of the screening process are shown as examples. mESC, mouse embryonic stem cell; p.i, post-infection; RVFV, Rift Valley fever virus. Scale bar: 0.5 mm.