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. 2023 Apr 18;6(7):e202302005. doi: 10.26508/lsa.202302005

Figure S7. LRP1 expression in wt and LRP1-depleted human cell lines.

Figure S7.

(A) LRP1 mRNA levels in WT and knockdown Calu-3 cells as measured by RT–qPCR (samples of Fig 6A). Cells were infected with SARS-CoV-2 at an MOI of 0.1 or MOI of 1, RNA was extracted at the indicated time points p.i., and RT–qPCR was performed to detect LRP1 and the GAPDH reference mRNAs. RNA levels in the CTRL mock-infected were set to 100%. (B) LRP1 protein levels in WT and knockout HuH-7 cells and knockdown Calu-3 cells as measured by immunoblot analysis. CTRL, control; NTC, no template control; WT, wild type.