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. Author manuscript; available in PMC: 2023 Apr 19.
Published in final edited form as: Methods Mol Biol. 2022;2374:1–11. doi: 10.1007/978-1-0716-1701-4_1

Figure 1: Diagram of experimental approach.

Figure 1:

Embryonic stem (ES) cells with distinct genotypes are harvested and mixed at appropriate ratios. The resulting aggregates, called mosaic embryoid bodies, contain Shh expressing, responding, and transporting cells, and can be differentiated into tissue resembling that of the developing spinal cord.