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. Author manuscript; available in PMC: 2023 Apr 19.
Published in final edited form as: Methods Mol Biol. 2022;2374:1–11. doi: 10.1007/978-1-0716-1701-4_1

Figure 2: Examples of mosaic SCOs.

Figure 2:

A: Shh−/− SCOs were generated with or without 3% of Shh-expressing cells (indicated). The presence of the Shh expressing cells (labeled for Shh in green) induces the localized expression of the motor neuron marker Isl1 (magenta). B: Ptch1LacZ/LacZ;Ptch2−/−;Shh−/− mES cells were loaded with CellTracker™ CMFDA (green) dye and Shh−/− (left panel) or Shh−/−;Sc5d−/− (bottom row) mES cells with CellTracker™ CMAC (magenta) prior to aggregation. Images were taken at the indicated time points of aggregation. Earliest aggregates can be observed approximately 15 h after cells were mixed and placed on a rotating platform.