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SCOs start expressing either ventral or dorsal markers of neural progenitor cells depending on their genotype and environment. These markers can be stained with fluorescently labeled antibodies (or fluorescent reporters) at the indicated recommended time points of in vitro culture.
| identity | neural marker | recommended SCO incubation time |
|---|---|---|
| ventral | Nkx2.2 | 4 days |
| Olig2 | 4 days | |
| Isl1/2 | 4–5 days | |
| Hb9 | 5 days | |
| Sox2 | 3 days | |
| dorsal | Pax7 | 2 days |
