Table 2. Primers for qPCR Analysis.
| target genes | primer sequence | annealing temp. (°C) | purposes | refs |
|---|---|---|---|---|
| attB-upstream | 5′-GCCGACAACAAAGTCAGGTT-3′ | 59.5 | detecting E. coli DNA | (85) |
| 5′-AAAAGAAGCGCAGAATTTCG-3′ | ||||
| attB-attP | 5′-AGACGGGAAACTGAAAATGTG-3′ | 59.5 | detecting the integration of λ phage genome | (85) |
| 5′-CTGATAGTGACCTGTTCGTTGC-3′ | ||||
| traI | 5′-TTGAACTCTGCTGTGCCGTTGAC-3′ | 58.0 | transcriptomic analysis of conjugation | a |
| ATCACGAAGAAGGGAACCATCATC-3′ | ||||
| traJ | 5’-GACGTGCTCATAGTCCA-3′ | 58.0 | a | |
| 5’-TGTACTGCCTTCCAGAC-3′ | ||||
| trfAp | 5′-GAAGCCCATCGCCGTCGCCTGTAG-3′ | 58.0 | (84) | |
| 5′-GCCGACGATGACGAACTGGTGTGG-3′ | ||||
| lamB | 5′-GGTGGTTCTTCCTCTTTC-3′ | 59.0 | transcriptomic analysis of phage delivery assays | a |
| 5′-CGACACCCAGTTCTAATG-3′ | ||||
| malT | 5′-GCAGGCCGGACGTAAAAGTGAC-3′ | 59.0 | a | |
| 5′-ATGCTGTTCCAGTTCCGGCA-3′ |
a
Primers were custom designed and ordered from Integrated DNA Technology (IDT, USA).