Brain and blood concentrations of DOI after a single administration (2 mg/kg) in male (n = 4) and female (n = 3–4) C57BL/6J mice. A) Frontal cortex concentrations of DOI 0.5, 1, and 4 h after a single administration. B) Blood concentrations of DOI 0.5, 1, and 4 h after a single administration. Two-way ANOVA followed by Bonferroni’s post hoc test (*P < 0.01, ***P < 0.001). Data show mean ± S.E.M.