Figure S3.

FACS gating for blood analysis from chimeric mice. Complete FACS gating for blood lineage output from chimeric mice. First, cells were gated based on SSC/FSC, and then single cells were selected. CD150^−/− and wt cells were separated by gating on, respectively, CD45.1- and CD45.2-positive cells. In each, lymphoid cells were isolated by gating on CD11b-negative cells; then, B cells were CD19-positive cells, and T cells were CD3ε-positive cells. In Cd11b-positive cells, granulocytes and monocytes are distinguished by gating onto, respectively, Ly6G-positive and -negative cells.