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. 2023 Apr 17;220(7):e20221649. doi: 10.1084/jem.20221649

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Figure S2. — GRB2 and THEMIS cooperate to inactivate SHP1. (A) Protein expression in Ptpn6-tg (left) and Themis-tg (right) thymocytes. (B) Overexpression of THEMIS in CD2-iCre; Grb2f/f; Themis-tg mice results in an increase in phosphorylated SHP1. (C) Ptpn6 (Shp1) haploinsufficiency (±) partially rescues the developmental defect in Grb2-deficient thymocytes. Shown are phenotypes of thymocytes (left panels) and splenocytes (right panels) from the indicated mice. Numbers are percentage of cells within each gate. Data shown in B and C are representative of four mice for each genotype. For all experiments, efficient Cre-mediated deletion was confirmed by Western blot. Source data are available for this figure: SourceData FS2.