Figure 3.

CLH-6 is widely expressed and localizes to lysosomes. (A–F′) DIC and confocal fluorescence images of wild-type worms expressing CLH-6::GFP at different stages (A–C′) and in different tissues at the L4 stage (D–F′). White arrows indicate intestine and body wall muscle. The vulva region is surrounded by the dashed line. (G–K) Confocal fluorescence images of the hypodermis in wild-type adults co-expressing CLH-6::GFP or CLH-6::CHERRY with different organellar markers including NUC-1::CHERRY (lysosome, G–G′′), AMAN-2::mKate2 (Golgi, H–H′′), TagRFP-T::RAB-5 (early endosome, I–I′′), GFP::TRAM-1 (ER, J-J′′). White and blue arrowheads indicate structures labeled by CLH-6 and organellar markers, respectively, and the purple arrowheads indicates co-localization of CLH-6 and NUC-1::CHERRY. Quantifications are shown in K. At least 10 animals were scored in each strain, and data are shown as mean ± SD. Scale bars: 5 µm.