Figure 4.

Loss of clh-6 affects degradation of endocytic and autophagic cargoes. (A–I) Confocal fluorescence images of the hypodermis in wild-type (A–A′′, D–D′′, G–G′′) and clh-6(qx604) (B–B′′, E–E′′, H–H′′) adults expressing NUC-1::CHERRY and DPY-7::GFP (A–B′′), GFP::LGG-1 (D–E′′) or SQST-1::GFP (G–H′′). Arrowheads indicate DPY-7–, LGG-1–, and SQST-1–positive structures that contain faint NUC-1::CHERRY fluorescence. Quantification analyses are shown in C, F, and I. At least 15 animals were scored in each strain. (J and K) Western blot analysis of LGG-1-I and LGG-1-II (lipid-conjugated form) in wild type, clh-6(qx604), and clh-6(tm617). LGG-1 accumulation was quantified and normalized to onefold in wild type (K). At least three independent experiments were performed. In C, F, I, and K, data are shown as mean ± SD. Student’s two-tailed unpaired t test was performed to compare mutant datasets with wild type. **P < 0.001, ***P < 0.0001. Scale bars: 5 µm. Source data are available for this figure: SourceData F4.