Figure 5.

Blocking endocytosis or autophagy suppresses lysosome integrity defects in clh-6 mutants. (A–R) Confocal fluorescence images of the hypodermis in the indicated strains expressing GFP::Gal3 without treatment (A–F and L–Q), or with DMSO treatment (H and J), or with Pitstop 2 treatment (I and K). The experiments using the temperature-sensitive dyn-1(ky51) allele were performed at 25°C, which is the non-permissive temperature of the mutant allele. The average number of GFP::Gal3-positive membrane-like structures in the indicated strains is quantified in G and R. Scale bars: 5 µm. In I and K, 17 animals (n) were scored in each strain. In G and R, data are shown as mean ± SD. Unpaired two-tailed Student’s t test was performed to compare double mutant datasets with clh-6(qx604) worms. 10 animals were scored in each strain. ***P < 0.0001.