Fig. 4.

Cellular responses to treatment with Ad5-RBD administered intranasally (i.n.) or intramuscularly (i.m.) in HLA-DQ6 mice. Groups of female HLA-DQ6 mice (n = 3) were inoculated once with Ad5-RBD at doses ranging from 10⁵-10¹⁰ viral particles/mouse (a, b). Groups of male or female HLA-DQ6 mice were inoculated once or twice (14 days apart) with Ad5-RBD at doses 10⁷ or 10⁹ viral particles/mouse (c). After 25–28 days, spleen cells were restimulated in vitro with recombinant SARS-CoV-2 spike protein receptor binding domain (RBD). Supernatants were harvested after 3 days, and the effector proteins IFNg and granzyme B were measured by ELISA. Results expressed as concentrations (pg/ml). Statistical comparisons were performed using Kruskal-Wallis test and Dunn’s test for multiple comparisons. Group differences were non-significant (adjusted p-values > 0.05).