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. 2023 Apr 14;41(20):3233–3246. doi: 10.1016/j.vaccine.2023.04.020

Fig. 5.

Fig. 5

Transcriptomic profiles of RBD-stimulated spleen cells from mice treated with Ad5-RBD administered intranasally (i.n.) or intramuscularly (i.m.). Groups of female HLA-DQ6 mice (n = 3) were inoculated once with Ad5-RBD at doses ranging from 105-1010 viral particles/mouse. After 25 days, spleen cells were restimulated in vitro with recombinant SARS-CoV-2 spike protein receptor binding domain (RBD). Cells were harvested after 3 days of culture, and bulk RNA was isolated. Heatmaps depicting the up- (a; red color, 86 genes) or down-regulation (b; blue color, 62 genes) of altogether 148 genes differentially expressed after treatment with Ad5-RBD in at least one of two separate comparisons, and are ordered to show the genes with the highest log2 fold changes from the top [Ad5-RBD at doses 105/106 viral particles i.n. (ineffective treatment) vs. doses 107/108 viral particles i.m. or doses 109/1010 viral particles i.n. (n = 6; adjusted P value, P ≤ 0.001; RNA sequencing)]. Statistical analyses were performed using edgeR. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)