Figure 2.

Depletion of T_regs during neoadjuvant ICB induces the systemic expansion and activation of T cells and NK cells.

(A) Schematic overview of intervention study using orthotopically transplanted KEP tumors. (B-E) Absolute cell counts of CD8+ T cells (B), NK cells (C), CD4+ T_helper cells (D), and CD4+CD8+ double-positive T cells (E) in blood of mice treated as indicated, determined by flow cytometry pre-mastectomy (n = 5-8). (F-G) Frequency of indicated markers (% of positive cells) gated on CD8+ T cells (F) or CD4+ T_helper cells (G) in blood of mice treated as indicated, determined by flow cytometry pre-mastectomy (n = 5-8). (H) Frequency of PD-1+ CD44+ CD62L− effector CD8+ and CD4+ T cells in the blood of mice treated as indicated, analyzed by flow cytometry pre-mastectomy (n = 5-8). Data in B-H show mean ± SEM. P-values were calculated by One-way ANOVA with Sidak’s correction (B-E,H) or Two-way ANOVA with Sidak’s correction (F,G). ns, not significant, * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.