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. 2022 Sep 8;146(4):1496–1510. doi: 10.1093/brain/awac326

Figure 4.

Figure 4

Effect of ptpa knock-down on D. melanogaster negative geotaxis measured by the climbing test. Comparative climbing test of three different UAS constructs (prkn, ptpa, nsmase) with and without L-DOPA treatment. prkn is used as positive control (significant locomotor impairment). nsmase is used as a negative control (no locomotor impairment). Climbing test performed at Days 10, 20, and 35 represented as proportion of flies that did not reach the 5 cm threshold. No significant difference is observed in locomotor behaviour between ptpa and controls on Day 10. Significant locomotor defect was observed at Days 20 and 35 for the ptpa and prkn lines compared to the negative control nsmase. This defect was significantly corrected with L-DOPA treatment. Significant comparisons are indicated by an asterisk. *P < 0.05. Human and Drosophila orthologues: PRKN (human)/prkn (D. melanogaster); PTPA (human)/ptpa (D. melanogaster); SMPD2 (human)/nsmase (D. melanogaster).