Skip to main content
. Author manuscript; available in PMC: 2023 Apr 19.
Published in final edited form as: Nat Plants. 2022 Nov 10;8(11):1289–1303. doi: 10.1038/s41477-022-01270-3

Extended Data Fig. 5 |. Expression of RRT1, RRT2, RRT3, RRT4, and co-expression of RRT1:RRT2.

Extended Data Fig. 5 |

Four proteins from the RRT family were expressed in HEK293 cells. A co-expression experiment in which RRT1 and RRT2 were co-transfected into the cells was also performed. Total protein is the measure of fluorescence in the cells + culture medium. Secreted protein is the measure of fluorescence in cell-free medium. Of the four RRT-family proteins expressed in this system, RRT4Δ51 yielded the highest total protein. RRT4 protein expressed with 50% secretion efficiency. Error bars represent the standard deviation of two biological replicates. Co-expression of RRT1Δ61 with RRT2Δ62 did not result in increased expression, suggesting that these two proteins do not form a heterocomplex in vitro.