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. Author manuscript; available in PMC: 2023 Apr 19.
Published in final edited form as: Nat Plants. 2022 Nov 10;8(11):1289–1303. doi: 10.1038/s41477-022-01270-3

Extended Data Fig. 6 |. The purified RRT4 protein has RG-I:RhaT activity.

Extended Data Fig. 6 |

The purified RRT4 enzyme was incubated with 1 mM UDP-Rha and an RG-I (G) acceptor, DP12. Activity was tested at pH 6.5 and 7.0 with either 1 µM or 5 µM enzyme. The reaction progress was detected by MALDI-MS at the indicated time points. Activity at pH 6.5 was higher based on the relative conversion of the acceptor (2072 Da) to the RhaT product (2218 Da).