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. Author manuscript; available in PMC: 2023 Apr 19.
Published in final edited form as: Nat Plants. 2022 Nov 10;8(11):1289–1303. doi: 10.1038/s41477-022-01270-3

Extended Data Fig. 7 |. Individual RGGAT1 and RRT4 enzymes do not polymerize RG-I.

Extended Data Fig. 7 |

RGGAT1 enzyme (1 µM) was incubated with 100 µM RG-I (R) acceptor and 1 mM of UDP-GalA, UDP-Rha, or a combination of UDP-GalA and UDP-Rha. The activity was limited to addition of a single GalA residue with no additional products detected when UDP-Rha was included in the reaction. RRT4 enzyme (1 µM) was incubated with 100 µM RG-I (G) acceptor and 1 mM of UDP-GalA, UDP-Rha, or a combination of UDP-GalA and UDP-Rha. The activity was limited to addition of a single Rha residue with no additional products detected when UDP-GalA was included in the reaction.