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. 2023 Apr 19;14:2249. doi: 10.1038/s41467-023-37972-1

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Fig. 4 — a Maximum-likelihood phylogenetic tree of traced lineages containing IgM Rep-seq sequences: ADI-66196, ADI-67860, and ADI-67983. Germlines sequences were obtained from the IgBLAST-generated “germline_alignment” column of the sequence with the smallest IGHV SHM in the lineage. b Dot plot of % nucleotide IGHV SHM for the ADI-66196, ADI-67860, and ADI-67983 lineage sequences. Purple crossbars represent mAb values, while the blue crossbars represent average values of traced variants for each timepoint.