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. 2023 Mar 27;12:e81743. doi: 10.7554/eLife.81743

Figure 7. SNV-53 protects hamsters when given before or after HTNV inoculation.

(a) 8-week-old Syrian hamsters (n=8 per treatment group) were administered 5 mg/kg of the indicated Ab treatment and then inoculated 1 day later with 200 PFU of HTNV i.m. All animals were sacrificed at 28 dpi and organs were harvested. (b) Ab detection in the serum at day 0 by pseudovirion neutralization assay (PsVNA) and at day 28 by nucleoprotein ELISA. Dotted line indicated limit of detection. (c) qRT-PCR detection of HTNV genome in the lungs and kidneys. Kruskal-Wallis test with multiple comparisons of each group to vehicle, * p<0.01, ** p<0.001, *** p<0.001, **** p<0.0001. ns, not significant. Dashed line indicates the limit of detection, which was 50 copies of HTNV S-RNA per 100 ng/100 ng RNA.(d) In-situ hybridization detection of HTNV genome in the lungs and kidneys. One-way ANOVA with multiple comparisons of each group to vehicle, * p<0.01, ** p<0.001, *** p<0.001, **** p<0.0001. ns, not significant. (e) Eight-week-old Syrian hamsters (n=6 per treatment group) were inoculated with 10 PFU of HTNV i.m., and 5–10 mg/kg of indicated Ab was administered via i.p. route at 3 dpi. All animals were sacrificed at 28 dpi and organs were harvested. (f) Serum was analyzed as indicated in b. (g) Lung and kidneys were assayed as described in c.(h) Lung and kidneys were assayed as described in d.

Figure 7.

Figure 7—figure supplement 1. Pre- and post-exposure HTNV serum PsVNA50 data and pathology staining.

Figure 7—figure supplement 1.

(a) Neutralizing antibody levels in serum for the four treatment groups at days 0 or 28 measured by PsVNA50 from the pre-exposure HTNV study. (b) In situ hybridization (ISH) staining scores for individual animals from the pre-exposure HTNV study. (c) Representative ISH staining in lung and kidney sections. Slides were scored by intensity ranging from 0 to 3, with median values presented. (d) Neutralizing antibody levels in serum for the four treatment groups at days 4 or 28 measured by PsVNA50 from the post-exposure HTNV study. (e) in situ hybridization (ISH) staining scores for individual animals from the post-exposure HTNV study.