FIGURE 4.

HIF-1α is involved in the regulation of TRIM28 on DLL, Notch and VEGFR expression. A-C, The ECs were transfected with scrambled or TRIM28 siRNA alone or co-transfected with HIF-1α siRNA for 48 hours and then, cultured for 16 hours under normoxia or mild hypoxia (2% O₂) in Tri-gas CO₂ Incubators. The mRNA expressions of VEGF (A), VEGFR1 (B) and VEGFR2 (C) were quantified using qRT-PCR method. n = 3. **P < .01. D-K, ECs were transfected with either scrambled or TRIM28 siRNA alone, or co-transfected with HIF-1α siRNA for 48 hours. The mRNA expressions of DLL1 (D), DLL3 (E), DLL4 (F), and Notch1 (G), Notch2 (H), Notch3 (I), Notch4 (J) were analyzed using qPCR method. n = 4. *P < .05, ns., no significance. Total proteins in control or TRIM28 silenced cells were collected for western blotting assay (K). n = 3. The representative images were shown. Statistical significance was calculated by Tukey’s multiple comparison test