FIGURE 6.

VEGFA induces phosphorylation of TRIM28. ECs were starved in low-serum (1% FBS) medium overnight and then, treated with VEGF₁₆₅ (20 ng/mL) for 15 min. Immunoblot (A) and immunofluorescent (B) analysis were performed to examine the phosphorylation of TRIM28 (Ser473). Scale bar, 10 μm. n = 3. **P < .01. Statistical significance was calculated referring to the biological replicates by unpaired Student’s t test. C, The serum-starved ECs were incubated with DMSO, LY294002 (10μM), PD98059 (10 μM), or SB203580 (10 μM) for 10 minutes, and then, stimulated with VEGF₁₆₅ for another 15 minutes. Phosphorylated levels and total TRIM28, ERK, MAPKAPK-2, and AKT were examined using western blot. n = 3. The representative images were shown