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. 2023 Mar 29;616(7957):590–597. doi: 10.1038/s41586-023-05832-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — a, Gel filtration profile and Coomassie blue-stained SDS-PAGE of the GSDMB-IpaH7.8 complex. Results representative of more than 3 independent experiments. b, A brief flow chart of single-particle cryo-EM data collection and process. c, Cryo-EM map (Upper panel) and local resolution estimation (lower panel) of the GSDMB-IpaH7.8 complex calculated using ResMap. The highest resolution is observed at the IpaH7.8-LRR domain and GSDMB-N domain. GSDMB-C exhibits relatively low resolution. d, The gold-standard Fourier shell correlation (FSC) curve for the overall map and model-to-map correlations of the GSDMB-IpaH7.8 complex.