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. 2023 Apr 10;18(4):336–342. doi: 10.1038/s41565-023-01328-z

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© The Author(s) 2023

Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.

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Extended Data Fig. 1 — (a) Using the 120 nm nanorulers, we measured the distance between intensity peaks after expansion. (b) The mean distance (and SD) between 120 nm nanorulers was calculated, providing an average expansion factor of 3.7x (N = 50). The data is reported as mean ± SD. Then, we used nuclear stainings in tissues (c), the cross-sectional area of individual nuclei was calculated before and after expansion (d), providing an average expansion factor of 3.8x (N = 18).