Extended Data Fig. 5. Innate immune landscape during critical illness.

FlowSOM clustering of single-cell mass cytometry data was used to identify unique clusters of (a) neutrophil, (b) monocytes, (c) dendritic cell, and (d) NK cells in blood from critically ill patients (N = 51) sampled on day 1 of admission (N = 49), and again from survivors who remined in ICU on day 3 (N = 43), and day 7 (N = 15), compared to healthy volunteer controls (N = 12). Graphs shown log₂ fold difference of cluster abundance between healthy controls and ICU patients on ICU day 1, day 3, and day 7. Heatmaps show mean expression level of key markers for each cell type measured by mass cytometry (scaled by column/marker), as well as total quantity (mean) of each cell cluster (cells/mL of blood).