Extended Data Fig. 2. Safety evaluation of IT anti-PD1 in C57BL/6 mice.

Twenty mice were divided into three volume matched (6.5μl) treatment groups, 1). IT PBS (N=6); 2). IT isotype control (N = 7); 3). IT mouse anti-PD1 (13μg) (N = 7). Line graph plotting the mean weight of animals in the three IT treatment groups. Bars represent standard errors of the mean. No mice displayed morbidity/significant weight change during 14-day observation period. Note that additional animals were observed at 48 hours (PBS = 7; IT isotype control = 8; IT anti-PD1 = 8) and 7 day (PBS = 5; IT isotype control= 7; IT anti-PD-1 = 7) time points (prior to euthanasia for histological analysis) and no significant weight changes were observed at these time points.