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. Author manuscript; available in PMC: 2023 Apr 20.
Published in final edited form as: Sci Transl Med. 2022 Jun 29;14(651):eabm3151. doi: 10.1126/scitranslmed.abm3151

Fig. 3. Complement is associated with protection through lysis of virions and infected cells.

Fig. 3.

Pre-inapparent (blue dots) and pre-symptomatic (orange) secondary infection plasma samples were incubated with DENV3 virions in the presence of fresh guinea pig complement and RNase A (A-B). Virion lysis was determined by measuring viral RNA of intact virions by RT-qPCR (# of genome copies) and calculated as percentage (%) of lysis based on the average of five DENV-naïve plasma samples and one medium-only (no plasma) sample (DENV-naïve). A pool of DENV-immune plasma samples was used as positive control (DENV-immune pool). C) Antibody-dependent complement-mediated lysis (ADCML) of BHK-21 cells was detected by measuring the optical density (OD) values associated with lactacte dehydrogenase (LDH) release in the supernants of mock- or DENV3-infected cells incubated with the plasma samples and fresh exogenous human complement. Non-parametric Mann-Whitney t test, *p<0.05; ns, non-significant. Data represents average of experiments done in technical triplicates.