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. 2023 Apr 19;14:2248. doi: 10.1038/s41467-023-38014-6

Fig. 5. The properties of NIL-IM-Lip + L on the ICD effect, DC maturation and the killing effects of NK cells in vitro.

Fig. 5

a Fluorescence microscopy images of CRT exposure to B16F10 cells. The IR780 + L, IR780 + 1-MT + L, IR780 + 1-MT + IL-15 + L and NIL-IM-Lip+L groups were irradiated with an 808 nm laser for 5 min (1.0 W/cm2) (n  =  3 biologically independent experiments). b Schematic illustration of the ICD effect to induce tumour cell death, including CRT exposure, HMGB1 release, and ATP secretion, which could promote the maturation of DCs. c Fluorescence microscopy images of HMGB1 release from B16F10 cells. The IR780 + L, IR780 + 1-MT + L, IR780 + 1-MT + IL-15 + L and NIL-IM-Lip+L groups were irradiated with an 808 nm laser for 5 min (1.0 W/cm2) (n  =  3 biologically independent experiments). d Flow cytometric analysis of CRT exposure to B16F10 cells (n  =  3 biologically independent experiments). e Quantitative ELISA analysis of HMGB1 release into the B16F10 culture medium supernatant (n  =  3 biologically independent experiments). f Quantitative ELISA analysis of ATP secretion into the B16F10 culture medium supernatant (n  =  3 biologically independent experiments). g Schematic illustration of the transwell system used for DC maturation detection. h, i, Flow cytometric analysis of the ratio of mature DCs after coculture with B16F10 cells prestimulated with different formulations (n  =  3 biologically independent experiments). j Quantitative ELISA analysis of TNF-α secretion in the DC culture medium supernatant (n  =  3 biologically independent experiments). k Schematic illustration of the experimental process to determine the killing ability of NK cells. l, B16F10 cells killed by NK cells after pretreatment with different formulations (n  =  3 biologically independent experiments). Data are presented as mean values ± SD. Statistical significance was calculated by the one-way ANOVA analysis of variance with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001, ns P > 0.05. Source data are provided as a Source Data file. ns not significant.