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. 2023 Apr 19;14:2248. doi: 10.1038/s41467-023-38014-6

Fig. 7. Effects of NIL-IM-Lip + L treatment on the simultaneous coactivation of CTLs and NK cells and the reversion of the suppressive conditions in the TLIME in the B16F10 model.

Fig. 7

a–c Flow cytometric analysis of intratumoural CD4+ T cells and CD8+ T cells. d Flow cytometric analysis of the infiltration of CTLs into tumours (n  =  3 biologically independent experiments per group). e, f Flow cytometric analysis of NK cell infiltration into the tumours (n  =  3 biologically independent experiments per group). g Flow cytometric analysis of the infiltration of Treg cells gated on CD4+ lymphocytes in the tumours (n  =  3 biologically independent experiments per group). h Quantitative ELISA analysis of the cytokines in the tumour tissues from mice treated with different formulations (n  =  3 biologically independent experiments per group). i–k Flow cytometric analysis of the infiltration of CD4+ T cells and CD8+ T cells in the LNs (n  =  3 biologically independent experiments per group). l, m Flow cytometric analysis of NK cell infiltration into LNs (n  =  3 biologically independent experiments per group). n Flow cytometric analysis of the infiltration of Treg cells gated on CD4+ lymphocytes in the LNs (n  =  3 biologically independent experiments per group). o, p Flow cytometric analysis of DC infiltration in LNs (n  =  3 biologically independent experiments per group). Data are presented as mean values ± SD. Statistical significances were calculated by one-way analysis of variance with Tukey’s post hoc test. *P < 0.05, **P < 0.01, ***P < 0.001. Source data are provided as a Source Data file. N-I-Lip+L NGR-IR780-Lip+Laser, NIL-I-Lip+L NGR/IL-15-IR780-Lip+Laser, IL-IM-Lip+L IL-15-IR780/1-MT-Lip+Laser.