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. 2023 Apr 19;14(4):278. doi: 10.1038/s41419-023-05800-5

Fig. 7. Both NCAM1 overexpression and NCAM1 mimetic peptide FGL rescue KLK8-overexpressed HT22 cells from apoptosis.

Fig. 7

HT22 murine hippocampal neuronal cells were infected with KLK8 adenovirus (Ad-KLK8) at a multiplicity of infection (MOI) of 3 for 24 h with or without NCAM1 adenovirus (Ad-NCAM1) (A–D) or NCAM1 mimetic peptide FGL (E–H). A, B CCK8 assay and measurement of caspase-3 activity showed that Ad-NCAM1 blocked Ad-KLK8-induced cell injury and caspase-3 activation in a dose-dependent manner in HT22 cells, respectively. C Protein levels of Bcl-2 and Bax were determined by western blot analysis. D Showed representative TUNEL-stained (red) cells. Nuclei were counterstained with DAPI (blue). Scale bar = 100 μm. E, F CCK8 assay and measurement of caspase-3 activity showed that FGL peptide blocked Ad-KLK8-induced cell injury and caspase-3 activation in a dose-dependent manner in HT22 cells, respectively. G protein levels of Bcl-2 and Bax were determined by western blot analysis. H Showed representative TUNEL-stained (red) cells. Nuclei were counterstained with DAPI (blue). Scale bar = 100 μm. Data were presented as means ± SD (n = 4, one-way ANOVA, Bonferroni’s post hoc test). *p < 0.05, **p < 0.01.