Figure 3.

SARS-CoV-2 epitope recognition by peripheral blood mononuclear cells (PBMCs) from the patient

(A) Reaction of PBMCs to the peptide pools derived from proteins of the SARS-CoV-2 Wuhan strain. PBMCs collected on day 308 were stimulated with peptide pools for 48 h, and IFN-γ expression was determined using an ELISPOT assay. SARS-CoV-2 proteins with mutations detected in the patient are indicated.

(B) Concentrations of IFN-γ in the supernatant of PBMCs stimulated with SARS-CoV-2 peptides. PBMCs collected from the patient on day 324 were stimulated with the indicated individual peptides from the SARS-CoV-2 Wuhan-Hu-1 strain (Wu) for 12 days before being washed and re-stimulated with nothing (−), the same Wu peptides, or corresponding mutant peptides (Mut) for 20 h. Error bars indicate mean ± SD of duplicates. Data are representative of three independent experiments. See also Figure S2 and Table S8.