A) Representative images goosecoid gene expression in mink1 crispants injected with WT, S18D, or S43D HMGA2 mRNA. 4 biological replicates B) Quantification of pitx2c expression in mink1 crispants injected with WT, S18D, or S43D HMGA2 mRNA. 4 biological replicates P < 0.0001= ****, p < 0.0005= ***, p < 0.005= **, p < 0.05= * by two-tailed T-test.