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. 2021 Sep 23;32(17-18):959–974. doi: 10.1089/hum.2019.284

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© Pavel Marichal-Gallardo et al., 2021; Published by Mary Ann Liebert, Inc.

This Open Access article is distributed under the terms of the Creative Commons License [CC-BY] (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Figure 3. — CPSF6 knockdown by transduction with AAV vectors at several stages of their purification with SXC. U87 cells were transduced with different AAV vectors (wt AAV6 and DJP2) encoding a NS shRNA with a CFP reporter or a combination of three anti-CPSF6 shRNAs (shCPSF6a&b). (A) CFP-positive cells and CFP expression levels. (B) CPSF6-positive cells and CPSF6 expression levels. (C, D) Transduction of monocyte-derived macrophages from two different donors with SXC-purified AAV particles (Elution 1 from panels A and B) carrying an (C) NS shRNA or (D) three shRNAs targeting CPSF6. Data represent means of analytical replicates (n = 18) ± SE. Lamin A/C is a protein marker for the nuclear membrane. Scale bars in cell pictures represent 5 μm. CFP, cyan fluorescent protein; CPSF6, cleavage and polyadenylation specificity factor subunit 6; NS, nonsilencing; wt, wild type.