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. 2023 Apr 6;11:1063250. doi: 10.3389/fpubh.2023.1063250

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Copyright © 2023 Roggan, Kronenberg, Wollert, Hoffmann, Nisar, Konda, Diegeler, Liemersdorf and Hellweg.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Exposure to X-rays did not induce cell cycle arrests. Astrocytes were exposed to 8 Gy X-rays or mock-irradiated (0 Gy) and trypsinized and fixed at different time points up to 24 h after irradiation. The percentage of cells in the different cell cycle phases—G₁- (A), S- (B), G₂-phase (C)— was determined by flow cytometry of DAPI stained cells. The distribution of cells in S- and G₂-phase is comparable for both conditions. The samples were compared by unpaired t-test with a sample size of n = 6. Data are shown as mean ± SD. Significant differences in comparison to the 0 Gy control at the same time point are indicated by asterisks (*p < 0.05).