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. Author manuscript; available in PMC: 2023 Apr 20.
Published in final edited form as: Nat Microbiol. 2023 Mar 23;8(4):679–694. doi: 10.1038/s41564-023-01344-8

Extended Data Figure 3. Quality check of HCoV-OC43 infection and Poly (I:C) treatment in A549-ACE2 cells.

Extended Data Figure 3.

a. To examine how many cells were infected by HCoV-OC43 at 24hpi, immunostaining using a monoclonal antibody targeting HCoV-OC43 was conducted (MAB9012). Here we show representative confocal images of Mock and HCoV-OC43 infected A549-ACE2 cells stained with MAB9012 (red) and DAPI (blue). Scale bar is shown.

b. Barplots showing quantification of infection rates of HCoV-OC43 at 0.1 and 0.5 MOIs (24hpi). Data shows n = 5 for each quantification.

c. Barplots of DDX58, IFTI1, IFNB1 gene expression (RT-qPCR) showing known antiviral responses in poly(I:C) transfected cells. n = 3, representative of two independent experiments, P = 5.59e-5 (DDX58), 2.31e-4 (IFIT1), 5.94e-5 (IFNB1), calculated with a two-sided independent t-test. Data show Mean +/− SD from replicates (***, p<0.001).