KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| α-mouse CD8 | BD Pharmingen™ | Clone 53-6.7 Catalog # 553030 RRID: AB_394568 |
| α-mouse CD4 | BD Pharmingen™ | Clone GK1.5 Catalog # 553730 RRID: AB_396634 |
| α-mouse CD3 | BD Pharmingen ™ | Clone 17A2 Catalog # 560590 RRID: AB_1727461 |
| α-mouse CD45 | BioLegend™ | Clone 30-F11 Catalog # 103114 RRID: AB_312979 |
| α-mouse H2b | BioLegend™ | Clone AF6-88.5 Catalog # 116516 RRID: AB_1967133 |
| α-mouse H2b | BioLegend™ | Clone AF6-88.5 Catalog # 116520 RRID: AB_2721684 |
| α-mouse H2d | BioLegend™ | Clone SF1-1.1 Catalog # 116606 RRID: AB_313741 |
| α-mouse RORγT | BD Pharmingen ™ | Clone Q31-378 Catalog # 562894 RRID: AB_2687545 |
| α-mouse T-bet | BD Pharmingen ™ | Clone 4B10 Catalog # 561264 RRID: AB_10563424 |
| α-mouse Foxp-3 | BioLegend™ | Clone 150D Catalog #320008 RRID: AB_492980 |
| α-mouse CD45.1 | Invitrogen™ | Clone A20 Catalog # 12-0453-81 RRID: AB_465675 |
| α-mouse CD45.2 | Invitrogen™ | Clone 104 Catalog # 17-0454-81 RRID: AB_469399 |
| α-mouse IFN-γ | BD Horizon ™ | Clone XMG1.2 Catalog # 563376 RRID: AB_2738165 |
| α-mouse IL-17A | BioLegend™ | Clone TC11-18H10.1 Catalog # 506908 RRID: AB_536010 |
| α-mouse IL-10 | BioLegend™ | Clone JES5-16E3 Catalog # 505008 RRID: AB_315362 |
| α-mouse GM-CSF | Invitrogen™ | Clone MP1-22E9 Catalog # 12-7331-82 RRID: AB_466205 |
| α-mouse CD16/32 Block | BD Pharmingen™ | Clone 2.4G2 Catalog # 553142 RRID: AB_394656 |
| α-mouse CD44 | BioLegend™ | Clone IM7 Catalog # 103012 RRID: AB_312963 |
| α-mouse CD62L | BioLegend™ | Clone MEL-14 Catalog # 104406 RRID: AB_313093 |
| α-mouse Lag-3 | BioLegend™ | Clone C9B7W Catalog # 125221 RRID: AB_2572080 |
| α-mouse Lag-3 | BioLegend™ | Clone C9B7W Catalog # 125210 RRID: AB_10639727 |
| α-mouse Tim-3 | BioLegend™ | Clone B8.2C12 Catalog # 134004 RRID: AB_1626177 |
| α-mouse Tim-3 | BioLegend™ | Clone B8.2C12 Catalog # 134010 RRID: AB_2632734 |
| α-mouse Salmf6 | BioLegend™ | Clone 330-AJ Catalog #134610 RRID: AB_2728155 |
| α-mouse PD-1 | BioLegend™ | Clone 29F.1A12 Catalog #135231 RRID: AB_2566158 |
| α-mouse PD-1 | BioLegend™ | Clone 29F.1A12 Catalog #135220 RRID: AB_2562616 |
| α-mouse/human Galectin-3 | Biolegend™ | Clone eBioM3/38 Catalog # 12-5301-82 RRID: AB_842792 |
| α-human CD8 | BD Horizon ™ | Clone RPA-T8 Catalog # 751518 RRID: AB_2875513 |
| α-human CD4 | Invitrogen™ | Clone S3.5 Catalog # MHCD0430 RRID: AB_10371907 |
| α-human CD3 | BD OptiBuild ™ | Clone SK7 Catalog # 740202 RRID: AB_2739952 |
| α-human CD45RA | BD Horizon™ | Clone HI100 Catalog #568712 RRID: NA |
| α-human CD45RO | BD OptiBuild | Clone UCHL1 Catalog # 748367 RRID: AB_2872786 |
| α-human IFN-γ | BioLegend™ | Clone 4S.B3 Catalog # 502539 RRID: AB_11218602 |
| α-human IL-17 | BD Pharmingen™ | Clone N49-653 Catalog # 560491 RRID: AB_1645418 |
| α-human IL-10 | BD Horizon ™ | Clone JES-9D7 Catalog # 566276 RRID: AB_2738566 |
| α-mouse Foxp-3 | BD Pharmingen™ | Clone 150D Catalog # 560401 RRID: AB_1645201 |
| α-mouse Foxp-3 | BioLegend™ | Clone 150D Catalog # 320008 RRID: AB_492980 |
| α-human PD-1 | BioLegend™ | Clone EH12.2H7 Catalog # 329920 RRID: AB_10960742 |
| α-human CXCR-3 | BioLegend™ | Clone G025H7 Catalog # 353708 RRID: AB_10983064 |
| α-human CCR7 | BioLegend™ | Clone G043H7 Catalog # 353230 RRID: AB_2563630 |
| α-human TIGIT | BioLegend™ | Clone A15153G Catalog #372716 RRID: AB_2632931 |
| α-human Tim-3 | BioLegend™ | Clone F38-2E2 Catalog # 345026 RRID: AB_2565717 |
| α-human CD27 | BioLegend™ | Clone M-T271 Catalog # 356410 RRID: AB_2561957 |
| α-human CD127 | BD Horizon ™ | Clone HIL-7R-M21 Catalog # 565185 RRID: AB_2739099 |
| α-human Lag-3 | BioLegend™ | Clone 11C3C65 Catalog # 369320 RRID: AB_2716125 |
| Anti-mouse IFN-γ (Armenian hamster IgG) | BioXcell | Clone H22 Catalog # BE0312 RRID: AB_2736992 |
| Isotype (Armenian hamster IgG) | BioXcell | Clone BE0091 Catalog # BE0091 RRID: AB_1107773 |
| Ultra-LEAF™ Purified anti-mouse CD3ε Antibody | BioLegend™ | Clone 145-2C11 Cat# 100340 RRID: AB_11149115 |
| Ultra-LEAF™ Purified anti-mouse CD28 Antibody | BioLegend™ | Clone 37.51 Cat# 102116 RRID: AB_11147170 |
| Ultra-LEAF™ Purified anti-human CD3 Antibody | BioLegend™ | Clone OKT3 Cat# 317326 RRID: AB_11150592 |
| Ultra-LEAF™ Purified anti-human CD28 Antibody | BioLegend™ | Clone CD28.2 Cat# 302934 RRID: AB_11148949 |
| Biological Samples | ||
| allo-HCT patient colon biopsy | Roswell Park Comprehensive Cancer Center | N/A |
| Peripheral blood mononuclear cells from healthy donors | Roswell Park Database and Biorepository | N/A |
| Chemicals, peptides, and recombinant proteins | ||
| Recombinant Mouse IFN-γ (carrier-free) | BioLegend™ | Cat# 575304 |
| Cell Activation Cocktail (with Brefeldin A) | BioLegend™ | Cat# 423303 |
| Brefeldin A | BioLegend™ | Cat# 420601 |
| eBioscience™ Cell Proliferation Dye eFluor™ 670 | Invitrogen™ | Cat# 65-0840-85 |
| Percoll PLUS density gradient media | Cytiva | Cat# 17544502 |
| Critical commercial assays | ||
| “The Big Easy” EasySep Magnet | StemCell Technologies | Cat# 18001 |
| FOXP3 Fix/Perm Kit | BioLegend™ | Cat# 421401 |
| CellTrace™ Violet Cell Proliferation Kit | Invitrogen™ | Cat# C34557 |
| LIVE/DEAD™ Fixable Aqua Dead Cell Stain Kit | Invitrogen™ | Cat# L34957 |
| Mouse CD90.2 MicroBeads | Miltenyi Biotec | Cat# 130-121-278 |
| Mouse Pan T cell isolation Kit II | Miltenyi Biotec | Cat# 130-095-130 |
| FITC-Annexin V Apoptosis Detection Kit | BioLegend™ | Cat# 640922 |
| CaspGLOW™ Fluorescein Active Caspase-8 Staining Kit | Invitrogen™ | Cat# 88-7005-42 |
| BD Fixation/Permeabilization Kit | BD Biosciences | Cat # 554714 |
| Deposited data | ||
| Preclinical T cell RNA-sequencing dataset | ArrayExpress | Array Express: E-MTAB-5378, E-MTAB-5379, E-MTAB-5380, E-MTAB-5381 |
| Raw and analyzed T cell microarray data | This paper | GEO: GSE225756 Figshare: https://doi.org/10.6084/m9.figshare.21970883, https://doi.org/10.6084/m9.figshare.21970886 |
| Experimental models: Cell lines | ||
| Human: THP-1 -Luc cell line | Provided by Dr. Francisco Hernandez-Ilizaliturri (Roswell Park Cancer Institute) | N/A |
| Human: Raji -Luc cell line | Provided by Dr. Dr. Francisco Hernandez-Ilizaliturri (Roswell Park Cancer Institute) | N/A |
| Mouse: A20- Luc | Provided by Dr. Xuefang Cao (University of Maryland) | N/A |
| Experimental models: Organisms/strains | ||
| Mouse: C57BL/6J (B6) | The Jackson Laboratory | JAX# 000664 |
| Mouse: Gal-3−/− (C57BL/6J) | Gal-3−/− generated by the lab of Dr. Fu-Tong Liu through Dr. Noorjahan Panjwani (Tufts University). | N/A |
| Mouse: BALB/cJ | The Jackson Laboratory | JAX# 000651 |
| Mouse: C3H/SW | The Jackson Laboratory | JAX# 000438 |
| Mouse: CD45.1 C57BL/6J (Pep boys) | The Jackson Laboratory | JAX# 002014 |
| Mouse: NSG-HLA-A2 | The Jackson Laboratory | JAX# 014570 |
| Software and algorithms | ||
| GraphPad Prism_V9 | Graphpad | https://www.graphpad.com/scientific-software/prism/ |
| FlowJo™_V10 | FlowJo | https://www.flowjo.com/solutions/flowjo |