Key resources table
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| rat anti-HA | Roche | 3F10 |
| rabbit anti-PH3 | Millipore Sigma | 06–570 |
| chicken anti-GFP | Invitrogen | A10262 |
| mouse anti-Pnt | Christian Klambt lab | N/A |
| Bacterial and virus strains | ||
| Pseudomonas entomophila | Edgar lab | N/A |
| Biological samples | ||
| Chemicals, peptides, and recombinant proteins | ||
| Phusion DNA polymerases | Thermo Scientific | F530 |
| Collagenase I | Gibco | 17018029 |
| Propidium Iodide | Invitrogen | P1304MP |
| Hoechst 33342 | Thermo Scientific | 62249 |
| MitoTracker Deep Red | Invitrogen | M22426 |
| Tetramethylrhodamine | Invitrogen | T668 |
| 2NB-DG | Invitrogen | N13195 |
| Etomoxir | Cayman Chemicals | 11969 |
| human EGF | PeproTech | AF-100–15 |
| Binimetinib | LC Laboratories | N/A |
| MitoView™ 650 | Biotium | 70075 |
| JC-1 | Invitrogen | T3168 |
| Critical commercial assays | ||
| Arcturus picoPure RNA Isolation Kit | Applied Biosystems | Cat#12204–01 |
| QBT™ Fatty Acid Uptake Assay Kit | Molecular Devices | PN:R8132 |
| QIAamp DNA Micro Kit | Qiagen | Cat#56304 |
| Illumina TruSeq Stranded mRNA Kit | Illumina | 20020594 |
| TruSeq RNA Single Indexes Set A | Illumina | 20020492 |
| TruSeq DNA PCR-Free Sample Preparation kit | Illumina | FC-121–3001 |
| Deposited data | ||
| mRNA Sequence, DamID Sequence, and analyzed sequencing data | This paper | https://www.ncbi.nlm.nih.gov/geo/query/acc.cgi?acc=GSE181583 |
| Experimental models: Cell lines | ||
| Human RPE-1 cell | ATCC | CRL-4000 |
| Experimental models: Organisms/strains | ||
| D. melanogaster: esgts: esg-Gal4/Cyo; tubGal80ts UAS-GFP/TM6B | Bruce Edgar Lab | BAE418 |
| D. melanogaster: esgts F/O: esg-Gal4 tubGal80ts UAS-GFP/Cyo; UAS-flp>CD2>Gal4/TM6B | Bruce Edgar Lab | BAE419 |
| D. melanogaster: esg ts ; Su(H)-Gal80: esg-Gal4 UAS-2XEYFP; Su(H)GBE-Gal80 tub-Gal80 ts | Bruce Edgar Lab | CZ16 |
| D. melanogaster: W 1118 | Bloomington DSC | BL3605 |
| D. melanogaster: UAS-sSpi | Bloomington DSC | BL63134 |
| D. melanogaster: UAS-PntP1 | Bloomington DSC | BL869 |
| D. melanogaster: UAS-PntP2 | Bloomington DSC | BL399 |
| D. melanogaster: UAS-Ets21C-PC | FlyORF | F000624 |
| D. melanogaster: UAS-Krn | FlyORF | F002754 |
| D. melanogaster: UAS-Egfr-RNAi | Bloomington DSC | BL31525 |
| D. melanogaster: UAS-Pnt-RNAi | Bloomington DSC | BL31936 |
| D. melanogaster: UAS-Pnt-RNAi | Bloomington DSC | BL35808 |
| D. melanogaster: UAS-Ets21C-RNAi | VDRC | KK103211 |
| D. melanogaster: UAS-MEK-RNAi | VDRC | KK107276 |
| D. melanogaster: UAS-stg-RNAi (II) | Kyoto Stock Center | 1395R-1 |
| D. melanogaster: UAS-mtTFB2-RNAi | Bloomington DSC | BL27055 |
| D. melanogaster: UAS-TFAM-RNAi 1 64 | Kyoto Stock Center | 4217R-2 |
| D. melanogaster: UAS-TFAM-RNAi 2 | VDRC | 37819 |
| D. melanogaster: UAS-TFAM-RNAi 3 | VDRC | 10719 |
| D. melanogaster: UAS-mito-HA-GFP (II) | Bloomington DSC | BL8442 |
| D. melanogaster: UAS-mito-HA-GFP (III) | Bloomington DSC | BL8443 |
| D. melanogaster: UAS-Dam-Ets21C-PC | This paper | CZ39 |
| D. melanogaster: UAS-Dam-PntP1 | This paper | JYH182 |
| D. melanogaster: UAS-Dam-PntP2 | This paper | JYH183 |
| D. melanogaster: UAS-Ets21C-PB (II) | This paper | CZ51 |
| D. melanogaster: UAS-Ets21C-PC (II) | This paper | CZ22 |
| D. melanogaster: UAS-UAS-mtTFB2-Flag-HA | This paper | CZ229 |
| Oligonucleotides | ||
| For Dam-PntP1 vector, PntP1 start: CCTC GAG ATGCCGCCCTCTGCGTTTTT. Templet cDNA from Drosophila midgut | This paper | N/A |
| For Dam-PntP2 vector, PntP2 start: CCTC GAG ATGGAATTGGCGATTTGTAAAAC. Templet: cDNA from Drosophila midgut | This paper | N/A |
| For Dam-PntP1 and Dam-PntP2 vectors, Pnt stop: CTCT AGA CTAATCCACATCTTTTTTCTCAA. Templet: cDNA from Drosophila midgut | This paper | N/A |
| For Dam-Ets21C-PC vector, Ets21C-PC start: CCTC GAG ATGGCCATTCTACAGAATAGCC. Templet: cDNA from Drosophila midgut | This paper | N/A |
| For Dam-Ets21C-PC vector, Ets21C stop: CTCT AGA TCAGTTGAATGCATTTGTGGTG. Templet: cDNA from Drosophila midgut | This paper | N/A |
| For UAS-Ets21C-PB vector, Ets21C PB start: ATGAGCGTCAGCGTGGACGTG. Templet: cDNA from Drosophila midgut | This paper | N/A |
| For UAS-Ets21C-PC vector, Ets21C PC start: ATGGCCATTCTACAGAATAGCC. Templet: cDNA from Drosophila midgut | This paper | N/A |
| For UAS-Ets21C-PB and Dam-Ets21C-PC vectors, Ets21C rev (without stop codon): GTTGAATGCATTTGTGGTGG. Templet: cDNA from Drosophila midgut | This paper | N/A |
| For Mito-DNA content assay, Ets21C Forward: CCCTGACTATCTCGGGTGAA | This paper | N/A |
| For Mito-DNA content assay, Ets21C Reverse: CACTTCACTTTGGCCCTGTT | This paper | N/A |
| For Mito-DNA content assay, β-tub56D Forward: ACATCCCGCCCCGTGGTC | This paper | N/A |
| For Mito-DNA content assay, β-tub56D Reverse: AGAAAGCCTTGCGCCTGAACATAG | This paper | N/A |
| For Mito-DNA content assay, CO1 Forward: TGACTTCTACCTCCTGCTCT | This paper | N/A |
| For Mito-DNA content assay, CO1 Reverse: GCAATTCCAGCGGATAGAGG | This paper | N/A |
| For Mito-DNA content assay, CO3 Forward: TCACAGAAGTTTATCACCCGC | This paper | N/A |
| For Mito-DNA content assay, CO3 Reverse: TGGTGGGCTCAAGTTACAGT | This paper | N/A |
| Recombinant DNA | ||
| The UAS controlled overexpression vector for mtTFB2: UAS-mtTFB2-Flag-HA | Drosophila Genetics Resource Center | UFO03038 |
| Software and algorithms | ||
| Prism | GraphPad | SCR_002798 |
| Fiji | https://imagej.net | SCR_002285 |
| Bowtie 2 | http://bowtie-bio.sourceforge.net/ | SCR_016368 |
| STAR | https://github.com/alexdobin/STAR | SCR_004463 |
| HTSeq | https://pypi.org/project/HTSeq/ | SCR_005514 |
| Bedtools | https://github.com/arq5x/bedtools2 | SCR_006646 |
| R | www.r-project.org/ | SCR_001905 |
| Python | www.python.org/ | SCR_008394 |
| GSEA | www.gsea-msigdb.org/ | SCR_003199 |
| MetaboAnalyst | www.metaboanalyst.ca/ | SCR_015539 |
| Other | ||