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. 2022 Oct 31;14(11):mjac060. doi: 10.1093/jmcb/mjac060

Figure 4.

Figure 4

Active proviruses are excluded from HIV-1 MLOs. (A) Confocal images of THP-1 cells and primary MDMs expressing OR-GFP infected with HIV-1 ANCH3 (THP-1: MOI = 20, 3 days p.i.; MDMs: MOI = 40, 4 days p.i.). Analysis was performed from 3D acquisitions. (B) Confocal images of immuno-RNA FISH in primary MDMs infected with HIV-1 GFP (MOI = 20) and treated with or without NEV (10 µM) 2–3 days p.i. The scatter plots show the GFP intensity per cell ± SD and the percentage of vRNA foci outside CPSF6 clusters per nucleus ± SD (number of cells: 22 (–NEV), 20 (+NEV); number of vRNA foci: 268 (–NEV), 66 (+NEV)); Unpaired t-test, ****P ≤ 0.0001. (C) Correlation of GFP intensity and the percentage of vRNA foci excluded from CPSF6 clusters in HIV-1-infected cells (22 cells). Pearson's r coefficient, **P ≤ 0.01. (D) vRNA intensity per CPSF6 cluster per condition ± SD; unpaired t-test, **P ≤ 0.01. All the analyses were performed in 3D from two independent experiments. Scale bar, 5 µm.