KEY RESOURCES TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
|
| ||
| Mouse monoclonal anti-Tubulin beta-III | R&D | Cat# MAB1195; RRID:AB_356859 |
| Rabbit monoclonal anti-Isletl | Abcam | Cat# ab178400 |
| Chicken polyclonal anti-MAP2 | Abcam | Cat# ab5392; RRID:AB_2138153 |
| Rabbit monoclonal anti-Synapsin | Millipore | Cat# AB1543, RRID:AB_2200400 |
| Mouse monoclonal anti-Neurofilament-H, nonphosphorylated (SMI-32) | BioLegend | Cat# 801702, RRID:AB_2715852 |
| Goat polyclonal anti-Choline Acetyltransferase | Millipore | Cat# AB144P; RRID:AB_11212924 |
| Rabbit polyclonal anti-Foxpl | Abcam | Cat# ab16645, RRID:AB_732428 |
| Mouse moloclonal anti-Ankyrin-G | Millipore | Cat# MABN466, RRID:AB_2749806 |
| Guiena pig polyclonal anti-Synaptophysin | Synaptic Systems | Cat# 101 004, RRID:AB_1210382 |
| Mouse monoclonal anti-PSD-95 | Abcam | Cat# ab2723, RRID:AB_303248 |
| Mouse monoclonal anti-PSD-95 | Neuromab | Cat# 75-028, RRID:AB_2292909 |
| Mouse monoclonal anti-GAPDH | Millipore | Cat# MAB374, RRID:AB_2107445 |
| Rabbit polyclonal anti-SCG10/STMN2 | Novus Biologicals | NBP49461 |
|
| ||
| Bacterial and virus strains | ||
|
| ||
| FUW-TetO-Ngn2-Puro | (Zhang et al.)12 | N/A |
| FUW-rtTA | (Zhang et al.)12 | N/A |
|
| ||
| Chemicals, peptides, and recombinant proteins | ||
|
| ||
| Retigabine | Santa Cruz Biotechnology | N/A |
|
| ||
| Critical commercial assays | ||
|
| ||
| miRNeasy Mini Kit | Qiagen | 217,004 |
| iScript kit | BioRad | N/A |
| SYBR Green | BioRad | N/A |
| mini-PROTEAN® TGX™precast protein gels | BioRad | N/A |
| IRDye® secondary antibodies | Li-Cor | N/A |
| Axion Biosystems Multi-Electrode Array (MEA) plate system | Axion Biosystems | N/A |
|
| ||
| Deposited data | ||
|
| ||
| Raw and analyzed data | GEO: GSE219112DUOS: DUOS-000121 | https://duos.broadinstitute.org/ |
|
| ||
| Experimental models: Cell lines | ||
|
| ||
| Embryonic stem cell collection | Embryonic stem cell collection | Embryonic stem cell collection |
| Hues3-Hb9:GFP | HSCRB, Harvard University. (Di Giorgio, et al.)29 | N/A |
|
| ||
| Oligonucleotides | ||
|
| ||
| Pre-design qPCR Assays | IDT | https://www.idtdna.com/site/order/qpcr/predesignedassay |
|
| ||
| Software and algorithms | ||
|
| ||
| ImageJ-Fji | NIH | https://imagej-nih-gov.ezp-prod1.hul.harvard.edu/ij/, Fiji, RRID: SCR_002285 |
| GraphPad Prism | GraphPad software | https://www.graphpad.com; GraphPad Prism, RRID: SCR_002798 |
| Image Studio Lite Software | Image Studio Lite Acquisition Software LI-COR Biosciences | https://www.licor.com/bio/image-studio/; RRID: SCR_013715 |
| Seurat | (Hao and Hao et al.)66 | https://satijalab.org/seurat/index.html |
| BioRender | BioRender | http://biorender.com; RRID: SCR_018361 |