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. 2022 Dec 29;32(9):1511–1523. doi: 10.1093/hmg/ddac306

Figure 3.

Figure 3

Effects of p.G64R-DOK7 on protein expression and AChR clustering in C2C12 cells. (A) Representative western blotting and quantitative analysis of DOK7 in C2C12 cells transfected with wild-type (WT)-DOK7 or p.G64R-DOK7. Mean and SD (n = 3 experiments) are indicated with individual values in red dots. Student’s t-test (**P < 0.01). (B) Transfection of wild-type (WT)-DOK7 but not p.G64R-DOK7 induced AChR clustering visualized by Alexa 594-conjugated α-bungarotoxin (red) in C2C12 cells without agrin. Scale bar = 50 μm. (C) Quantitative analysis of the number, total area, average length and total signal intensity per visual field (0.143 mm2) of AChR clusters in C2C12 cells expressing WT-DOK7 or p.G64R-DOK7. p.G64R-DOK7 markedly reduced AChR clustering. The mean values of 30 visual images are indicated by red dots. One-way ANOVA and Dunnett’s multiple comparison test (ns, no significance; *P < 0.05, **P < 0.01 and ***P < 0.001).