Figure 7.

Conservation analysis, functional prediction and operon assignment for 48 novel small open reading frames (sORFs) of Sinorhizobium meliloti 2011. The conservation analysis was conducted using tBLASTn. The respective hits (see methods for parameters and cutoffs) are broadly summarized at the level of different taxonomic groups. The number of species outside the lower taxonomic unit, which harbors a hit, is given, if at < 10. In addition, the method by which the respective sORF was detected or confirmed is shown (Ribo-seq: ribosome profiling, MS: proteomics, WB: Western blot), as well as the results of predictions for membrane localization (by TMHMM and PSORTb), signal peptide II cleavage sites of lipoproteins (by LipoP), and function (by Phyre2; only hits with confidence levels greater than 30% are shown). For details on Phyre2 prediction and genomic context including operon prediction, see Table S8 and Table S9. sORF1 to sORF55 are a subset of the Ribo-seq-detected, translated sORFs, which are listed in Table S7, and sORF56 to sORF66 represent the novel sORFs identified by proteomics. sORFs encoding small proteins below 30 amino acids are shown in red. The putative sORF64, present in tmRNA, contains the proteolytic tag sequence. The sORF65 corresponds to the N-terminal HmuP extension; outside of Proteobacteria, it is conserved in many genera of Planctomycetes. *Structural genomics (92% confidence homology to protein of unknown function).