Figure 3.

Bacterial burden in animals infected with different A(H3N2) IAV strains 5 days prior to SPN challenge. Ferrets were infected with either A/Udorn/307/1972 or A/Sydney/1234/2019 (10⁵ TCID₅₀) in 500 µl PBS and monitored daily. A total of 5 days after viral infection, ferrets were inoculated with 10³ CFU of SPN in 50 µl of PBS. (A) Survival over time following bacterial inoculation. (B) Nasal wash samples were taken daily and the bacterial load determined on HBA plates. (C) Following euthanasia, blood was collected and the bacterial load determined. Bars represent the mean bacterial titre (± SD) and symbols show titres in individual animals.