Table 1.

Confirmed interactions between CdaR, CdaA, GlmM in Firmicutes.

Organism	Analyzed protein pair	Interaction/methods	Effect on c-di-AMP synthesis	References
B. subtilis	CdaR-CdaA	Yes/BTHa and activity assay	Activation, in vivo (tested in Escherichia coli)	Mehne et al. 2013, Gundlach et al. 2015a
	GlmM-CdaA	Yes/BTH assay, SPINEb, FPLCc, SAXSd and structure analysis, activity assay	Inhibition, in vitro	Gundlach et al. 2015b, Pathania et al. 2021
L. monocytogenes	CdaR-CdaA	Yes/BTH assay, activity assay	Inhibition, in vivo (tested in E. coli and L. monocytogenes)	Rismondo et al. 2016, Gibhardt et al. 2020
	GlmM-CdaA	Yes/BTH assay, pulldown assay, SEC-MALSe, ITCf, activity assay	Inhibition, in vivo (tested in E. coli and in L. monocytogenes) and in vitro	Gibhardt et al. 2020
L. lactis	CdaR-CdaA	Activity assay	No effecth	Zhu et al., 2015
	GlmM-CdaA	Yes/BTH assay,	Inhibition in vivo (tested in E. coli and L. lactis)	Zhu et al., 2015
S. aureus	CdaR-CdaA	Activity assay	Inhibition in vivo (tested in E. coli)	Zhu et al., 2015
	GlmM-CdaA	SAXSd, SECg, pulldown assay, activity assay	Inhibition in vivo (tested in E. coli) and in vitro	Tosi et al. 2019

^aBacterial two-hybrid assay.

^bStrep-protein interaction experiment, in vivo crosslinking in combination with a pulldown assay.

^cFast protein liquid chromatography.

^dSmall-angle X-ray scattering.

^eSize-exclusion chromatography and multiangle light scattering.

^fIsothermal titration calorimetry.

^gSize-exclusion chromatography

^h cdaR is a pseudogene in the L. lactis strain MG1363 (Zhu et al. 2016).