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. Author manuscript; available in PMC: 2023 Aug 1.
Published in final edited form as: Conserv Genet. 2022 Apr 28;23(4):669–681. doi: 10.1007/s10592-022-01444-8

Fig. 6. Wyoming toad transcriptomes reveal a single group of MHC class IIβ sequences.

Fig. 6

Phylogenetic trees comparing Wyoming toad (Anabax) MHC class IIβ chain protein sequences to common toad (Bufbuf), cane toad (Rhimar), Xenopus laevis (Xenlae) and Xenopus tropicalis (Xentro). Trees were generated using alignments of (a) contiguous β1-β2 domains or by using individual protein domains; (b) β1, and (c) β2. Annotated alignments of individual (d) β1, and (e) β2 domains from Wyoming toad with other anuran species. Asterisks indicate putative binding sites as described by Lillie et al (2016). Circles at nodes indicate bootstrap support values (BSS) with filled black circles indicating BSS=100, gray circles indicating BSS values equal to or greater than 90 but less than 100, and white circles indicating BSS values greater than 70 but less than 90. Scale bar indicates substitutions over time.