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. 2023 Apr 20;18(4):e0284453. doi: 10.1371/journal.pone.0284453

Fig 1. Design and purification of obscurin RhoGEF fragments.

Fig 1

A, obscurin RhoGEF region. Exon boundaries and bioinformatic analysis and structure prediction based on amino acid sequence of human obscurin B isoform (NCBI ref. seq. NM_001098623). B, gel-filtration elution profile of DH domain fragment comprising residues 5681–5889. C, SDS-PAGE analysis of samples at different steps of the purification process of the DH5681-5889 fragment. D, 1D-NMR analysis of purified DH5681-5889 fragment. NMR spectrum exhibits wide peak dispersal and peaks below 0 ppm (black arrow), indicating that the protein is folded.