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. 2023 Apr 20;18(4):e0284453. doi: 10.1371/journal.pone.0284453

Fig 4. Phosphorylation of obscurin RhoGEF domains.

Fig 4

A, phosphorylation of different human obscurin RhoGEF domain combinations by different kinases. B, MST2-phosphorylation of an N- and C-terminally extended human DH domain and a zebrafish DH-PH fragment compared to human SH3-DH. C, Guanosine nucleotide exchange factor activity of obscurin RhoGEF (10 μM) fragments phosphorylated at Thr5798 towards RhoA, Cdc42, Rac1 and RhoQ/TC10. Black arrows indicate addition of buffer/GEF/EDTA. Data represent mean of n = 2–4 experiments.